whole blood column kit (Miltenyi Biotec)

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Miltenyi Biotec whole blood column kit
Whole Blood Column Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/whole+blood+column/Whole+Blood+Column+Kit/pm41478418-80-7-30
Average 93 stars, based on 54 article reviews

whole blood column kit - by Bioz Stars, 2026-10

93/100 stars

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Labeling:

Article Title: Combining magnetically isolated CD45 cells with serum maintains intact drug responsiveness for ELISpot analysis in clinical trials
Article Snippet: Briefly, 2 mL of whole blood was labeled with 100 μL StraightFrom CD45 MicroBeads (Miltenyi Biotec) and incubated for 15 min at 4 °C. .. The labeled blood was then loaded onto a whole blood column (Miltenyi Biotec) placed in a MACS separator magnet (Miltenyi Biotec) and washed, and labeled cells were eluted with elution buffer. ..

Magnetic Cell Separation:

Incubation:

Article Title: Rapid and Direct Quantification of Viable Candida Species in Whole Blood by Use of Immunomagnetic Separation and Solid-Phase Cytometry
Article Snippet: Thirty microliters of polyclonal anti- C. albicans antibody conjugated with fluorescein isothiocyanate (FITC) (Acris Antibodies, Herford, Germany) and 30 μl monoclonal anti-FITC antibody bound to microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) were added to an EDTA-treated whole-blood sample (maximum volume, 15 ml). .. After incubation at room temperature for 1 h with head-to-tail rotation, the sample was loaded on a whole-blood column (Miltenyi Biotec). .. Prior to loading, the column was inserted into a QuadroMACS cell separator (Miltenyi Biotec) and prewashed with 3 ml of separation buffer (phosphate-buffered saline [PBS], 0.5% bovine serum albumin, and 2 mM EDTA, pH 7.2).

Article Title: Rapid and Direct Quantification of Viable Candida Species in Whole Blood by Use of Immunomagnetic Separation and Solid-Phase Cytometry
Article Snippet: Thirty microliters of polyclonal anti-C. albicans antibody conjugated with fluorescein isothiocyanate (FITC) (Acris Antibodies, Herford, Germany) and 30 l monoclonal anti-FITC antibody bound to microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) were added to an EDTAtreated whole-blood sample (maximum volume, 15 ml). .. After incubation at room temperature for 1 h with head-to-tail rotation, the sample was loaded on a whole-blood column (Miltenyi Biotec). .. Prior to loading, the column was inserted into a QuadroMACS cell separator (Miltenyi Biotec) and prewashed with 3 ml of separation buffer (phosphate-buffered saline [PBS], 0.5% bovine serum albumin, and 2 mM EDTA, pH 7.2).

other:

Article Title: Fc-engineered antibodies leverage neutrophils to drive control of Mycobacterium tuberculosis
Article Snippet: Specifically, a Whole Blood Column (Miltenyi, 130-093-545) was attached to the MidiMacs (Miltenyi) and primed with 3mL of Separation Buffer (PBS with 0.5% BSA and 2mM EDTA).

Article Title: Fc-engineered antibodies promote neutrophil-dependent control of Mycobacterium tuberculosis
Article Snippet: Specifically, a Whole Blood Column (Miltenyi, 130-093-545) was attached to the MidiMacs (Miltenyi) and primed with 3 ml of separation buffer (PBS with 0.5% BSA and 2 mM EDTA).

Review